Hi Members, We gave the samples for sequencing & we have obtained the .fastq file & .fastq zip file. I want to assemble & annotate the sequences. It is low/moderate coverage sample. So what can I do next? I have windows computer. Is it enough? Please do not be irritated by the simplicity of the question! I have to learn & complete the project! There was an excel sheet containing the following information (What can I infer from this anybody)
Experiment Name MISEQ RUN 75
Workflow GenerateFASTQ
Application FASTQ Only
Assay TruSeq LT
Description P178_P128_P271_P150
Chemistry Default
Reads 301
Thanks Raghul
Mammalian genome de novo assembly with 301 reads of MiSeq data in fa format (no qualities?)? This sounds like Mission Impossible. Could you please tell the species' name, print out first few lines of your fa and fa zip files as well sizes of these files or number of lines. Thank you
Sorry it is a .fastq file