Question

Indels by Sanger

0

Entering edit mode

7.7 years ago

generoso.ianniciello • 0

Can I detect small indels from Sanger sequencing results? How to perform the analysis?

indels sanger capillary • 3.1k views

ADD COMMENT • link updated 7.7 years ago by trausch ★ 1.9k • written 7.7 years ago by generoso.ianniciello • 0

score 1 · Answer 1 · 2017-03-29

1

Entering edit mode

7.7 years ago

WouterDeCoster 47k

Since small indels will cause shifts in your chromatogram these can be detected. It might be a bit problematic to identify which event exactly causes the shift. Nice software is for example novosnp, developed a while ago in the department I'm working.

ADD COMMENT • link 7.7 years ago by WouterDeCoster 47k

score 0 · Answer 2 · 2017-03-29

0

Entering edit mode

7.7 years ago

h.mon 35k

There is also SeqDoC.

Staden - more specifically, the Gap4 module, which has a contig editor - may be used for variant calling, although it is not designed for this, and it a bit difficult to learn.

ADD COMMENT • link 7.7 years ago by h.mon 35k

score 0 · Answer 3 · 2017-03-30

0

Entering edit mode

7.7 years ago

trausch ★ 1.9k

Indigo can decompose heterozygous InDels in Sanger traces.

ADD COMMENT • link 5.2 years ago by trausch ★ 1.9k