Retrieving upstream and downstream intron coordinates
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7.6 years ago
ccag ▴ 60

I have a large bed file of drosophila (dm6) coordinates of select RNA regions I am interested in. If the coordinates encompass an exon (which many of them do) I would like to return the coordinates of the 5' and 3' introns. I know there must be an easy way to do this, but I am not very familiar with RNA-seq techniques.

Eventually I'd want to integrate this into a custom script. Thanks so much!

introns annotation RNA • 1.5k views
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Bedtools should help

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