Using the align() function of the Rsubread package; error: unrecognized option `--gtfFeature'
0
0
Entering edit mode
7.7 years ago
biomagician ▴ 410

Dear all,

I am using the bioconductor package Rsubread to align reads to a genome:

Rsubread::align(index="output/genome/ref/index/subread/celegans",
                readfile1='output/reads/trimmed/pooled/altadena1.fastq.gz',
                input_format="gzFASTQ",output_format="BAM",
                output_file='output/out2.bam',unique=TRUE,indels=5, nthreads = 8,
                useAnnotation = TRUE, 
                annot.ext = 'output/genome/ref/annotation/celegans.gtf', 
                isGTF = TRUE)

However, this gives me an error:

> rsession: unrecognized option `--gtfFeature'

Whereas this does not give me an error:

Rsubread::align(index="output/genome/ref/index/subread/celegans",
                readfile1='output/reads/trimmed/pooled/altadena1.fastq.gz',
                input_format="gzFASTQ",output_format="BAM",
                output_file='output/out2.bam',unique=TRUE,indels=5, nthreads = 8)

Has anybody come across this problem before?

Thanks a lot.

C.

rsubread subread ngs next-gen seq alignment • 2.8k views
ADD COMMENT

Login before adding your answer.

Traffic: 3099 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6