Hi, I want to do tf analysis using online tool kindly tell me how to do it: Steps of my work: 1)identification of degs using r (260 degs identified ) 2)func. Enrichment analysis 3)mirna analysis using online tool(4 degs). Q1> how to do tf analysis(source>h.sapiens), do i have to find out tfs and tf binding motifs of all degs which was identified in (step 1) or i have to identified degs of (step 3) ? Q2> how to find out the tfs and binding motifs of degs using online tool? i am following this topic from a paper : tf analysis Transcription factors are important regulatory elements for downstream genes. With the identified degs, exploring the targets of transcription factor may help disclosure the pathogenesis of gastric cancer. Here we analyzed the upstream sequences of degs and presented the tfs enriched with target degs. The results of tf Analysis is listed in table iv. A total of 20 tfs were identified, with ets1 as the most significant one." I want to use jaspar but i dont understand it, also i read its documentation.
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can u please specify the organism in your study? If plant you can use PlantTFDB
i mentioned it in the post also for homo sapiens and kindly tell me the stepwise procedure for this , its been very helpful