tajima D, nucleotide diversity and haplotype diversity

Question

[wanjahi.njau]

how do you compute tajima D, nucleotide diversity and haplotype diversity using VCF tools if you have data of 8 populations of 1 individual each

[guillaume.rbt]

Unfortunately I think your sample size isn't big enough to make those analysis. One individual per population isn't enough to have meaningful information on your populations.