Entering edit mode
7.6 years ago
User 4014
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40
Hi,
I received data for genome assembly. After using BBduk to trim adapters and low quality reads (Q25), I used FASTQC to check the data, and I am not sure if I should concern about K-mer content warnings since other indicators look just fine.
The FastQC webpage says ... "Libraries which derive from random priming will nearly always show Kmer bias at the start of the library due to an incomplete sampling of the possible random primers".
Do you think this could be the case if my library was prepared with Illumina Nano kit, size selected to 650 bp, and sequenced using HiSeq 2000 1*100 PE?
Thanks so much in advance and have a great day!
![http://imgur.com/wCeP7hP][1] ![http://imgur.com/tEhdGnn][2]
