Blastn stopped finishing commands it previously finished

Question

[Jacob]

I was running blastn on the command line queries like this one

blastn -query Acan -db nr -out "blastOutput/AcanHomo sapiens_blast.txt" -evalue 0.00001 -outfmt 6 -entrez_query "Homo sapiens[Organism] AND refseq[filter]" -remote

Were working fine. After I tried to run some commands like

nohup ./script.sh

screen ./script.sh

./script.sh ^Z bg disown %1

The query's no longer finished I also ran about 60 or so queries in a loop. Does blast have some sort of limit for people?

Thank-you

[h.mon]

Are you running more than one -remote instance at the same time? This may be the problem:

The BLAST+ remote service uses a shared resource (the computers at the NCBI), so only one BLAST+ application should run remote searches at a time.