Entering edit mode
8.0 years ago
landscape95
▴
190
Hi everyone, I have an miRNA expression matrix (in the picture below), when I try to normalize the data with TCGAanalyze_Normalization(miRNA_data, geneInfo)
I have some troubles
I Need about 0 seconds for this Complete Normalization Upper Quantile [Processing 80k elements /s]
Step 1 of 4: newSeqExpressionSet ...
Step 2 of 4: withinLaneNormalization ...
Error in names(y) <- 1:length(y) :
'names' attribute [2] must be the same length as the vector [0]
Timing stopped at: 0.004 0 0.003
I think of 2 problems here:
- I dont have the right "geneInfo" -> how can I get the right one?
- There seems to be the rows (which stands for genes) which seems like they are in the family of Accession numbers as I search on http://www.mirbase.org/cgi-bin/mature.pl?mature_acc=MIMAT0000062. Eg. "MIMAT0000062" owns Stem-Loops "hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3". I want to normalize data that rows for accession numbers and columns for samples. How can I solve this?
Your help is really appreciated!
Does anyone have a solution to this problem?
for gene info: https://github.com/BioinformaticsFMRP/TCGAbiolinks/issues/19. Look at the last comment. Once you have that you may need to match the miRNA list between your df and geneinfo.
Thanks for your reply. Both the workflow and datatype names had been changed in the package, so I needed to match those names. Works fine now.