How to match fixed number of data in one object with a data frame of a genome?
0
0
Entering edit mode
7.5 years ago
mkmansley • 0

Sorry for the basic question, it stems from a bigger issue I'm having with matching my RNAseq DE genes with the full list of annotated genes.

In this setting, I have pulled out the top 10 DE genes which I have stored as an object "top10". This is a list of the Ensembl identifiers e.g. ENSMUSGxxxx. I then have created an annotation data frame "ann" consisting of the 47,729 genes in the mouse genome which contains a "gene_id" column i.e. ENSMUSGxxxx and a gene name column "gene_name" i.e. Gnai3.

What I'm trying to do is to match my "top10" with the "gene_id" column in my object ann but also show the corresponding gene name. I'm sorry if this is simple, but I can't figure out the code.

I've tried many things, the last of which was trying to create a new object "keepgene":

keepgene <- (ann$gene_id == top10) But I get the error message "longer object length is not a multiplier of shoter object length".

I'd appreciate any help as I seem to have reached a sticking point!

Thanks very much,

Morag

RNA-Seq R • 1.1k views
ADD COMMENT
0
Entering edit mode

Try using ann$gene_id %in% top10

ADD REPLY
0
Entering edit mode

Thank you! I tried this and it returned many falses (47,719) and the 10 genes of interest. There is a maybe a way to then say to take the trues identified from that piece of code and then show their matching gene_name. I did it a different way where I used the merge function to merge the top10 object with a modified annotation object (ann), matching them by their row names. This seemed to do the trick!

ADD REPLY
0
Entering edit mode

Find yourself a basic R tutorial. I can't think of a good one ?which will tell you this off the top of my head

ADD REPLY

Login before adding your answer.

Traffic: 2272 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6