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7.6 years ago
kk.mahsa
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150
is there any way to figure out library preparation kit from fastq file (sequenced by illumina)? reads identifiers in fastq file can help me to recognize it?
I don't think so, but have a look:
https://support.illumina.com/help/SequencingAnalysisWorkflow/Content/Vault/Informatics/Sequencing_Analysis/CASAVA/swSEQ_mCA_FASTQFiles.htm
You can try to see by FASTQC tool what is the length of one read, if it's PE or SE, maybe there are some adaptors left - and try to fit with Illumina kits.
i did it but there is no adapter.
how your reads are named? Is it SE or PE? What is the length of read?
it is PE reads and 2*100
When you run fastQC it will also tell you which illumina technology was used (like illumina 1.9 or illumina 1.5+). This is not a clear answer but can allow you to search which are the library preparation kits for such technologies, perhaps there is only one (i'm guessing).