Non Linux software to variant sequences calling using fasta and VCF files

Question

[ehsansepahi]

I have a fasta file of my 270 short sequences and related VCF file. I want to call variants of a sequence using fasta and VCF file. there are some softwares that do this but those are linux based and I am not acquaintance with linux. Is there any windows, cloud or web based software for this?

This is an example of my interest: New Fasta Sequence From Reference Fasta And Variant Calls File?

[Devon Ryan]

The link in your post is for making a new fasta file from a VCF file, not calling new variants from a fasta file and a VCF file. Which do you actually want to do? If you really want to call variants using fasta and VCF files, then what role do you want the VCF file to play in this process?

[WouterDeCoster]

If you have a vcf file, you already have variants. So what exactly do you want to do?

[ehsansepahi]

regarding variants in VCF file and each sequence in reference fasta file, I want to have all possible sequences. for example:

sequence 1 is: ATCAGATCTA

VCF file says that in sequence 1 in position 4 "A" is replaced with "T" so the new sequence is ATCTGATCTA

I want a software that produce a fasta file containing both sequences. some thing like this:

sequence 1 var 1: ATCAGATCTA

sequence 1 var 2: ATCTGATCTA

[WouterDeCoster]

This is what you are looking for: A: New Fasta Sequence From Reference Fasta And Variant Calls File?

By limiting yourself to Windows you are also eliminating a lot of possibilities. If you want to do bioinformatics, Windows is not your friend.