Hello!
Recentlly, I trimmed my poor tail .fastq dataset using Trimmomatic's SLIDINGWINDOW parameter, in which I chose threshold value 15~30(i.e SLIDINGWINDOW:4:15~SLIDINGWINDOW:4:30). after, I mapped them using STAR, but I found with the increase of threshold, totally, unique and multiple mapped reads were all decreased across my 5 test_samples.
So, I'm in doubt with this situation, should it's a normal sense that my totally mapped or unique mapped reads increase with the accompany of increased trimming threshold?
I would really appreciate for your helping!
Thanks Brain! I'm sorry to mistaken replying a wrong place below instead of commmenting your answer.