How to get non-interacting protein pairs in Human

Question

[lakshmi.bioinformatics]

I am working on human protein protein interaction I need to run SVM I need a negative data set comprising of non interacting protein pairs how to generate these. I found this database Negatome (http://mips.helmholtz-muenchen.de/proj/ppi/negatome/) but the the problem is it talking about most mammals I cant restrict my search to human. Is there any other database containing human non interacting protein pairs or is there any other method to do this. Thanks in advance.

Answer 1

[Jean-Karim Heriche]

The Negatome database contain some human proteins which you can extract. In addition, there's this paper (and data here). Also if your protein interaction data is from AP-MS, you may want to make use of the CRAPome database.

Answer 2

[lakshmi.bioinformatics]

Thank you @Jean-Karim Heriche

My data is from online databases and its not an experimental one thanks again for the information. How to extract only the human Ids from Negatome

[Jean-Karim Heriche]

As far as I remember, the Negatome database uses UniProt accession numbers so you could use UniProt's REST API to figure out which species each protein belongs to. If you want to combine data sets from multiple sources, I would suggest to map the different IDs to the same human reference genome annotation e.g. EnsEMBL.

[Jean-Karim Heriche]

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