Entering edit mode
7.4 years ago
cpak1981
▴
140
I'm using stringtie
to assemble transcripts. I use stringtie
to make a .gtf
file, which outputs the following format: ... gene_id "X"; transcript_id "X"; FPKM "0.0111";...
. The point is that each entry (transcript/exon) has a FPKM value associated with it (in the Notes
field).
I also use stringtie
to assemble transcripts into ballgown
-compatible files. t_data.ctab
also provides FPKM values for each transcript.
My issue is that these FPKM values (for a specific transcript) do not match...has anyone else compared these values? Any idea why there's a difference? And which value I should trust?
Please provide examples of differences