How to set the blast database of TopHat-Fusion

Question

[shiyang93]

I am using tophat fusion. My RNA-Seq data are from human, do I need to download the other_genomic* and nt* database from blast database as suggested by the user manual of TOPHAT-Fusion? http://ccb.jhu.edu/software/tophat/fusion_tutorial.shtml Anyone who is familiar with tophat can tell me? Thanks!

[kylevoyto]

I have the same questions. Were you able to get TopHat-Fusion working with only the human_genomic* files?

Answer 1

[Istvan Albert]

Since nt also contains the human genomic data it sounds like the other databases would only be needed when processing data other than human.

[shiyang93]

Thank you very much! By the way, there are only human_genomic.00 -08, 10 - 16, 18 -21 on the blast database, no 09 and 17. I do not know if this human_genomic* database is intact. Could you give me some advice?