STAR/StringTie compatibility on tag HI in the BAM alignment file
0
0
Entering edit mode
7.4 years ago
Megan ▴ 50

Hi, I am trying to do transcript reconstruction and quantification using StringTie on BAMs generated by STAR. According to https://ccb.jhu.edu/software/stringtie/#install, modifying the tag HI in the BAM alignment file to start at 0 (as previously required with STAR produced alignment files) is no longer needed starting at v1.1.0 release 10/19/2015. I came across this after having generated BAMs using STAR with --outSAMattrIHstart 0 option. And StringTie finished successfully using the BAMs generated by STAR. I'm really concerned if there are any underlying mistakes doing so. Thanks!

STAR StringTie • 2.6k views
ADD COMMENT

Login before adding your answer.

Traffic: 1663 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6