Search Transcripts with Amino Acid

Question

[immy123]

I want to get the transcripts using HGVS nomenclature for BRAF.p:V600E (homo sapiens). I am first requesting for multiple types of sequence with this API call: http://rest.ensembl.org/sequence/id/ENSG00000157764.json?multiple_sequences=true;type=protein;

I am unsure on how to proceed to get the final output. I saw in this question: Amino Acid Change To Genomic Location that I can use http://rest.ensembl.org/map/translation/ENSP00000288602/600..600?content-type=application/json (mentioned in the second answer) to get the final output. However, I want to get the transcripts.

[LLTommy]

Could you make your question a bit more specific - this is really not a proper question. (e.g.: What is your input? What are you exactly looking for, want do you want to accomplish? Which species are we talking about?)

[immy123]

I am sorry for not elaborating. I will edit the question with more information.

Answer 1

[LLTommy]

Ah, ok. So you would be happy with this kind of result? (Inputs taking from your API call)

ENSP00000420119 <--> ENST00000497784 ENSP00000288602 <--> ENST00000288602 ENSP00000419060 <--> ENST00000496384

I am sure you can do it via the API, but I did it via SPARQL (Ensembl RDF), it's a relatively easy call and yes, you could also do it programmatically:

 SELECT ?transcript WHERE {?transcript <http://purl.obolibrary.org/obo/SO_translates_to> <http://rdf.ebi.ac.uk/resource/ensembl.protein/ENSP00000419060>}

I have to go now, but I can explain it further later on if your interested. Cheers.