Protein blastp .pin file warning

Question

[arunprasanna83]

Hello,

I have recently run a blastp. My database size was 1.6 GB. I ran my query and got the output (~300MB). However, I noticed that, the log file had warning lines ..Unable to open *.pin file.

My concern is that, is this a serious case? i.e would it have affected BLAST results ?

Thanks in Advance,

AP

[st.ph.n]

Post the commands you used to make the database, and the blastp command.

[Jean-Karim Heriche]

This could mean that the database was not found (check the path) or that it was not properly formatted (try running makeblastdb again).