What is the actual meaning/philosophy lying under following method to define a new molecular target ?
Selection of a new molecular target
The target region of HSP70 was obtained for P jirovecii by designing generic primers to the flanking regions of P carinii. Nucleotide sequences for all Pneumocystis types available from public data repositories were searched using BlastX 24 against the non-redundant protein database. Expressed sequence tag data were assembled into contigs using CAP3 prior to searching in order to remove redundancy and improve sequence accuracy. Sequences with high quality matches (bit score 60) to proteins from a broad spectrum of eukaryotic species were considered further (minimally: Caenorhabditis elegans, Drosophila melanogaster, Saccharomyces
cerevisiae, Schizosaccharomyces pombe and Neurospora crassa)
I've edited the text because your question was embedded in the method description. You should be more careful when copy/pasting :) If this method is taken from a paper, it may be helpful to provide the reference to give some context.
sorry, this is a method description which is decribed within this article http://thorax.bmj.com/content/thoraxjnl/63/2/154.full.pdf
looking frustrately to be able to get feedback
sincerely
Emre
I've moved this to a comment. Please don't create an answer unless actually answering the question. Use the "add reply" button to reply to a comment.
dear Jean
could you help me ? plzz :((