I have a set of micro array data set and have total number of genes 23233 and subset of two classes like A and B after DEG so i want to anaylsis these for MGI Do disease data set by Hypergeometric test so what i take total number of genes =23233 class A like 1801 class B like 1417 so what i did i used that particular disease count in total number of genes and class and used this formula population size P= 23233 number of success in population= 8 of that disease in (P=23233) and calssA is subset of P is S =1843 and sucess in class A is S =3 and hypergeometric test value is Probability of drawing 3 successes or more from a sample of 1843: P(X >= 3) = 0.020604834192780287. Probability of drawing exactly 3 successes from a sample of 1843: P(X = 3) = 0.018475183897383608
so my first question is is this is right method or values I am using second there most of the values less then 0.5 so they mostly are non signficant
