Question

What Tool Should I Use To Assemble Data Produced By A 454 Sequencer?

4

Entering edit mode

14.6 years ago

Cupton ▴ 80

What do you like for assembling paired-end #454 sequence data? (for 200kb assemblies)

assembly next-gen-sequencing • 6.4k views

ADD COMMENT • link updated 13 months ago by Ram 44k • written 14.6 years ago by Cupton ▴ 80

Ram · Answer 1 · 2010-04-27

4

Entering edit mode

14.6 years ago

Bioch'Ti ★ 1.1k

Hi Cupton,

Regarding the adjustment of the question, I would strongly advise you to read this webpage that summarize all the available (free and commercial) tools to assemble 454 data: http://seqanswers.com/forums/showthread.php?t=43

Regards.

"Previous answer" below:

Anyway, if you are looking for an open source 454 assembler, I think that MIRA3 is the best candidate! It is running under Linux/OsX

Check: http://www.chevreux.org/projects_mira.html

Otherwise, you can have a look to Galaxy, an interesting emerging online tool: http://main.g2.bx.psu.edu/

Regards.

ADD COMMENT • link updated 13 months ago by Ram 44k • written 14.6 years ago by Bioch'Ti ★ 1.1k

1

Entering edit mode

Can you do (de novo) assembly using Galaxy? Can't seem to find it...

ADD REPLY • link 13.8 years ago by lexnederbragt ★ 1.3k

0

Entering edit mode

Galaxy is not really a tool but a collection of tools, most of which are unrelated to assembly. You should specify what tool in Galaxy do you have in mind.

ADD REPLY • link 14.6 years ago by Istvan Albert 102k

Ram · Answer 2 · 2011-02-22

3

Entering edit mode

13.8 years ago

Ketil 4.1k

Generally, I'd use Newbler, which isn't open source, but comes with the 454 equipment. Some people claim Celera gives higher quality, but so far, results have been ambiguous. I'd be wary of the de Bruijn-based ones, where I have seen very mixed results.

I'd also try to verify the assembly in any way you can, mapping reads (preferably independent ones) back, mapping ESTs or BAC ends, etc, not just relying on statistics like n50.

ADD COMMENT • link updated 13 months ago by Ram 44k • written 13.8 years ago by Ketil 4.1k

1

Entering edit mode

Highly agree! You will have to compare and verify different assemblies. and when it comes to open-source I don't think I want to see Newbler's source code (I had a look once a while ago)

ADD REPLY • link 13.8 years ago by Michael 55k

Ram · Answer 3 · 2011-05-18

An interesting resource on this is Nick Loman's blog entry on assembling Ion Torrent data

http://pathogenomics.bham.ac.uk/blog/2011/05/first-look-at-ion-torrent-data-de-novo-assembly/

While not quite 454, the technology is similar in many respects.

Anyway, we got better results from Celera (approximately Newbler quality) than from CLC for 454 data, but your mileage may vary.