Samtools flagstat unpaired reads
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7.4 years ago
rbronste ▴ 420

Trying to quickly remove reads samtools flagstat deems as unpaired from BAM file? Thanks.

samtools BAM Bowtie2 • 3.4k views
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Have you searched Biostars (use external google search) for past threads? There has to be something available already.

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7.4 years ago

remove unpaired files = reads that are not part of a paired-end experiment ?

samtools view -f 1 -b -o out.bam in.bam
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Yes just for BAM files already processed for removal of duplicates (Picard) and ChrM and ChrUN reads.

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