Hi, everyone,
Bowtie has an XA tag (XA:i:<n>), and its definition is as follows according to the manual.
For a read with no reported alignments, <N>
is 0 if the read had no alignments. If [-m
] was specified and the read's alignments were supressed because the [-m
] ceiling was exceeded, <N>
equals the [-m
] ceiling + 1, to indicate that there were at least that many valid alignments (but all were suppressed). In [-M
] mode, if the alignment was randomly selected because the [-M
] ceiling was exceeded, <N>
equals the [-M
] ceiling + 1, to indicate that there were at least that many valid alignments (of which one was reported at random).
Does XA:i:0 mean unique mapping, and XA:i:1 mean two alignments?
Thanks a lot!