bowtie XA tag
0
0
Entering edit mode
7.3 years ago
biolab ★ 1.4k

Hi, everyone,

Bowtie has an XA tag (XA:i:<n>), and its definition is as follows according to the manual.

For a read with no reported alignments, <N> is 0 if the read had no alignments. If [-m] was specified and the read's alignments were supressed because the [-m] ceiling was exceeded, <N> equals the [-m] ceiling + 1, to indicate that there were at least that many valid alignments (but all were suppressed). In [-M] mode, if the alignment was randomly selected because the [-M] ceiling was exceeded, <N> equals the [-M] ceiling + 1, to indicate that there were at least that many valid alignments (of which one was reported at random).

Does XA:i:0 mean unique mapping, and XA:i:1 mean two alignments?

Thanks a lot!

bowtie • 1.9k views
ADD COMMENT

Login before adding your answer.

Traffic: 1611 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6