NGS DNA and RNA QC metrics from PGM/Ion Reporter
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7.3 years ago
genya35 ▴ 50

Hello,

I would really like to extract QC metrics such Average Base Coverage Depth, Total Mapped Reads, e.g. from either PGM that produces the calls or IonReporter that annotates the data. I was able to find a CSV file that is liked on a PGM that contains most Run and Sample DNA related metrics, however, I can't find any RNA related metrics. I don't see a way of extracting these data from Ion Reporter.

Could someone please suggest a way of extracting these DNA & RNA related metrics such as: Total RNA reads, Q20 Bases and Mean Read Length from a file that can be accessed either from PGM or Ion Reporter?

Thanks

next-gen • 1.3k views
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