Entering edit mode
7.3 years ago
Jacob
▴
10
I'm running command line tblastx for some human genes as the query sequences against the human genome as the target sequence. Almost all the searches are returning a query cover (qcovhsp) of 33%. I'm thinking that it is no coincidence that amino acids are 3 nucleotides long and that my query covers are ~ 1/3. Can anyone give me some insight as to why these values are being returned and not 100, as is returned when I use blastn?