Interproscan GO terms results explanation

Question

[konstantinkul]

Hi all,

I am new in InterPro scan as well as in genome annotation. I need to classify genes by GO terms to obtain a general table "GO Terms Classification Count Results" Could you please explain me one thing. When I run Interproscan with '-goterms' option it generated for me such output where several GO terms appear? So I need parsing this string to get only one or I need get all of them for further analysis (for instance https://www.animalgenome.org/bioinfo/tools/catego/) ? And what exactly means if several GOs appear?

H31_00826   08305e1960d087dfd44f6179ca32d6b9    88  SMART   SM01387     7   87  1.3E-47 T   21-08-2017  IPR000589   Ribosomal protein S15   GO:0003735|GO:0005622|GO:0005840|GO:0006412

Thank you in advance!

Answer 1

[Jean-Karim Heriche]

A given protein may be annotated with multiple terms from the same or multiple domains. In your example, the protein is annotated as being involved in translation (GO:0006412, biological process domain) and as being part of the ribosome (GO:0005840, cellular component domain). So if you want to understand your protein, you need to parse all these terms. Sometimes a protein may be annotated with both a term and one or more of its children (an ontology is a directed acyclic graph), in which case you may want to keep one or more depending on the level of specificity you need.

[konstantinkul]

Ok, I got it! Thanks a lot!

[WouterDeCoster]

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