PCR : genes vs pseudogenes

Question

[Kane1987]

Hi everyone,

I was wondering : How is it possible to be sure while doing a PCR to get the desired gene instead of a pseudogenes ???

Thanks for helping me out :)

Kane

Answer 1

[christopher medway]

There will be differences in the sequence - so you have to get clever when designing your primers. Probably not the best forum for a PCR design based question. Try Protocol Online or similar.

Answer 2

[Kane1987]

Thank you very much for your answer and the link. I'll ask there for details.