Entering edit mode
7.3 years ago
YOUSEUFS
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30
Hello Bioinformatics wizards!
I've recently counted my aligned reads w/ htseq-count and I am now placing them in excel next to their PROKKA gene ID's to see what genes these reads are actually aligning to. I'm noticing that out of the 1080 genes 37 of them are missing in my htseq-counts. Genes with 0 counts still show up. Is this to be expected? Have I fudged up somewhere? knowledge please!
Many thanks, bioinformatics peon
Does the gtf contain those genes?