Question

Is there any significant difference between endogenous/ native promoter and constitutive promoter (35S) on protein abundance?

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7.3 years ago

WUSCHEL ▴ 810

If a same gene is clone either endogenous promoter or constitutive promoter (35S), Do we get a significantly different protein abundance?

promoter genome gene expression • 2.5k views

ADD COMMENT • link updated 7.3 years ago by Jean-Karim Heriche 27k • written 7.3 years ago by WUSCHEL ▴ 810

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This is not a bioinformatics question and would be more appropriate to biology.stackexchange.

ADD REPLY • link 7.3 years ago by Jean-Karim Heriche 27k

score 0 · Answer 1 · 2017-08-23

This outcome is unpredictable as far as I know. This would depend on the relative "strength" of the two promoters, whether and how they would be regulated in the cell you're using. The type of expression/overexpression you're using is also a factor, e.g. if doing stable transfection with random integration in the genome, your clone will be subject to regulation by the chromatin environment it ends up in.