extract the aligned read names and their reference fasta sequence name
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7.3 years ago
trisha ▴ 10

Its a silly question
How to extract list of read names and their corresponding aligned fasta sequence name as table from the reference provided based on alignment.bam file

RNA-Seq alignment • 3.4k views
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Just in case your end goal is to find how many reads are aligned to each chromosome. You can just use:

samtools idxstats input.bam
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Thank you, this gives me the number of the reads mapped to each chromosome, However I would like to know the name of the read corresponding to its mapped chromosome
for example from your given samtools indexstats
chr1 38193400 19672 0
I would like to know the name of the reads that mapped to the chromosome one

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What about my suggestion?

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Thank you for your suggestion, However I get the following error. may be I did not understand correctly.
samtools view sorted_trimmed_corrected_merged.bam | cut -f1,3 Unless fasta identifier != "fasta sequence name"
cut: Unless: No such file or directory
cut: fasta: No such file or directory
cut: identifier: No such file or directory
cut: fasta sequence name: No such file or directory

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What?! But. The last sentence was not part of the command. You just need to run the following:

samtools view alignment.bam | cut -f1,3
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Thank you so much, I really apologies for this stupid blunder. This works perfectly well.

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Great, I have moved my comment to an answer so you can mark it as accepted and as such mark this question as solved.

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7.3 years ago

Probably I don't understand the question, but what about this?

samtools view alignment.bam | cut -f1,3

Unless fasta identifier != "fasta sequence name"

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Indeed, I may have missed the "name" part at the end (on my screen it's showing up on a separate line). I saw the title and thought that the sequence from the fasta file itself was what was needed.

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