Using multiple filters in BiomaRt's getSequence

Question

[spiral01]

Hi. I have a data frame of ensembl gene IDs and their corresponding canonical transcript IDs. Below is a small subset of this data frame:

             geneID    transcriptID
ENSG00000186092 ENST00000335137
ENSG00000187634 ENST00000420190
ENSG00000187642 ENST00000341290
ENSG00000188157 ENST00000379370
ENSG00000186891 ENST00000379265

I would like to retrieve the protein coding sequences for these genes using the R package biomaRt. Using the code below I can do this:

mart <- useMart("ensembl", dataset="hsapiens_gene_ensembl")
seq = getSequence(id =  "ENSG00000186092",
                  type = c("ensembl_gene_id"),
                  seqType = "coding",
                  mart = mart)

My issue is that I would like to add a second filter of transcript ID so that I am only getting the gene sequence for the specific transcript. However, I am not sure how to do this for the getSequence function. I have tried to add a value argument (as with getBM) but this gives the unused argument error message. Does anyone know how to do this?

Answer 1

[Emily]

Change your filter so that you're filtering by the Ensembl transcript ID:

seq = getSequence(id =  "ENST00000335137",
               type = c("ensembl_transcript_id"),
               seqType = "coding",
               mart = mart)

[spiral01]

Hi Emily, thanks that does help. However is it actually possible to use two different filters?

[Emily]

You can filter by multiple types of ID, but you can filter by a list of IDs, plus a biotype or something.

[deepue]

Hi Emily, did you mean "You can <<not>> filter by multiple types of ID, but ..." ?