fastq quality trimmer

Question

[abhilashreddy495]

#fastq_quality_trimmer -v -t 30 -z -i out2.fastq -o out2tt.fastq

this is the code i have run but my ngs data has 150bps after the code run this result is shown 120 bps.

#fastq_quality_trimmer: found empty nucleotide sequence on line 876486

what are the empty nucleotide sequence

[ATpoint]

A first approach would be to look at it by eye:

gzcat fastq.gz | sed -n 876486,876487p

Or sed directly, if not compressed.

[abhilashreddy495]

thank you there is a mistaken in the format