Tool for manual annotation curation
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7.3 years ago
Rox ★ 1.4k

Hello everyone,

I am looking for a visualization tool that will allow me to load different kind of features (a genome.fasta file, a annotation.gff/gtf file, bam an sam files...) but also to manually correct the annotation feature.

For example, if I see a gene structure that is not concordant with RNA-seq evidences, I want to manually correct the annotation file within the tool.

To do such a thing, I already heard about Apollo, but it seems really heavy to install as it's web based, and the desktop application is no longer supported. I've also heard about Artemis, but it is not specified if it allow manual modification of a gtf/gff file.

I'm currently using IGV to visualize my data, but I could never find a way to manually correct annotation file within IGV.

Does someone know about such a tool ?

Thanks for your help !

Roxane

annotation • 2.2k views
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Sounds like something that can be done in Artemis, did you try it?

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I never worked with Artemis, and before downloading and installing he tool, I wanted to be sure it could be done. I was reading the Artemis description, but didn't saw anything about manual genome curation.

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