About bedtools closest command line output understanding
0
0
Entering edit mode
7.3 years ago
bk11 ★ 3.0k

Hi, I used bedtools command to get nearest expressed genes and long non coding RNA and got result like this. I really did not understand the last column. Why distance is not matching?

Command used:

bedtools closest -a lincRNA.gtf -b *_lincRNAs.bed -d | head | less -S

Output:

1  432600  433992  ENSGALG00000036347  lincRNA +       1       1       531144  532064  .       .       +       Cufflinks       exon    .       gene_id "XLOC_000013"; transcript_id "TCONS_00000015"; exon_number "1"; oId "CUFF.10.1"; class_code "u"; tss_id "TSS13";        97153
RNA-Seq next-gen • 3.4k views
ADD COMMENT
0
Entering edit mode

Can you post a snippet of your GTF and BED files?

ADD REPLY
0
Entering edit mode
Hello James Ashmore,
Here are the snippets:
GTF:
1       432600  433992  ENSGALG00000036347      lincRNA +
1       698664  700562  ENSGALG00000033552      lincRNA +
1       754797  757791  ENSGALG00000034779      lincRNA +
1       787776  789085  ENSGALG00000034602      lincRNA +
1       1014153 1015081 ENSGALG00000043038      lincRNA +
1       1016111 1018736 ENSGALG00000008153      lincRNA +
1       1161094 1189206 ENSGALG00000042593      lincRNA -
1       1355907 1356649 ENSGALG00000039622      lincRNA +

BED:
1       531144  532064  .       .       +       Cufflinks       exon    .       gene_id "XLOC_000013"; transcript_id "TCONS_00000015"; exon_number "1"; oId "CUFF.10.1"; class_code "u"; tss_id "TSS13";
1       745809  746104  .       .       -       Cufflinks       exon    .       gene_id "XLOC_002014"; transcript_id "TCONS_00002492"; exon_number "1"; oId "CUFF.29.1"; class_code "u"; tss_id "TSS2192";
1       755070  755280  .       .       -       Cufflinks       exon    .       gene_id "XLOC_002016"; transcript_id "TCONS_00002494"; exon_number "1"; oId "CUFF.31.1"; class_code "u"; tss_id "TSS2194";
1       1304961 1305339 .       .       -       Cufflinks       exon    .       gene_id "XLOC_002040"; transcript_id "TCONS_00002519"; exon_number "1"; oId "CUFF.50.1"; class_code "u"; tss_id "TSS2218";
1       1305924 1306186 .       .       -       Cufflinks       exon    .       gene_id "XLOC_002041"; transcript_id "TCONS_00002520"; exon_number "1"; oId "CUFF.51.1"; class_code "u"; tss_id "TSS2219";
1       3031193 3031408 .       .       +       Cufflinks       exon    .       gene_id "XLOC_000057"; transcript_id "TCONS_00000070"; exon_number "1"; oId "CUFF.59.1"; class_code "u"; tss_id "TSS62";
1       3034131 3034340 .       .       +       Cufflinks       exon    .       gene_id "XLOC_000058"; transcript_id "TCONS_00000071"; exon_number "1"; oId "CUFF.60.1"; class_code "u"; tss_id "TSS63";
1       3034902 3035641 .       .       -       Cufflinks       exon    .       gene_id "XLOC_002049"; transcript_id "TCONS_00002531"; exon_number "1"; oId "CUFF.61.1"; class_code "u"; tss_id "TSS2228";
ADD REPLY

Login before adding your answer.

Traffic: 1928 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6