Miranalyser For Mirna Prediction

Question

[vandanabioinfo23]

How to make bowtie index from transcriptome sequence for miRNA prediction from miRAnalyser tool?

[David Langenberger]

Perhaps I could help you, but I don't get your question. Can you give some more details, what you have and what you want to have?

Answer 1

[sure]

I have not used miRAnalyser but if you want to make bowtie index for transcriptome sequences (fasta format), use bowtie-build function of bowtie (http://bowtie-bio.sourceforge.net/manual.shtml#the-bowtie-build-indexer). same is true if using bowtie2 instead of bowtie. General usage is bowtie-build [options]* <reference_in> <ebwt_base> You can use index generated in this way for any of the tools that uses bowtie.