Difference between unlocalized and unplaced fragments
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7.3 years ago
biomagician ▴ 410

Hi,

I am trying to scaffold 97 contigs from C. elegans into chromosomes. After blasting my contigs to the reference, I am running the chromosomer fragmentmap command:

chromosomer fragmentmap output/alignment/celegans/ref/pacbio/contigs/canu/blastn/bristol.txt 1000 output/assembly/celegans/canu/bristol/celegans.contigsChromosomer.fasta output/alignment/celegans/ref/pacbio/contigs/canu/blastn/map.txt
2017-08-30 17:30:13 - 59 mapped fragments of total length 58925391 bp
2017-08-30 17:30:13 - 38 unlocalized fragments of total length 43828989 bp
2017-08-30 17:30:13 - 0 unplaced fragments of total length 0 bp
2017-08-30 17:30:13 - 6 chromosomes formed
2017-08-30 17:30:13 - 59000 bp of gaps inserted

These contigs all have a pretty good primary alignment to the reference since they come from the reference strain. Therefore, I have quite high expectations in terms of forming complete chromosomes. Do you know what the difference is between the unlocalized and the unplaced fragments?

Thanks.

Best, C.

chromosomer scaffolding scaffold contig • 1.7k views
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