Weighed Reading Of An Electropherogram

Question

[Anima Mundi]

Hello,

I would like to know how to read an electropherogram in a "weighed" manner, that is: if I desired to obtain not a simple sequence from an electropherogram on the base of the better peak in each position, but rather a 2 lines matrix considering the 2 best picks*, what kind of application could I use?

*Each related to a probability depending on the peak's height.

PS: As some of you know, I am in wait of indications regarding the nick I am using. I will continue using the "wiee" nick only until I receive a communication. I do not want to disturb here and I would like to clarify the problem.

[Anima Mundi]

Finally I made the "metamorphosis", now I am Anima mundi. All fixed.

Answer 1

[Pierre Lindenbaum]

The staden package ( http://staden.sourceforge.net/ ) contains a 'C' library for reading the traces. As far as I remember, you'll get the intensities for each base and for each position. Are they normalized ? I don't know.

[Neilfws]

Anima wrote: Unfortunately I do not know nothing about normalization in electropherograms, as I am not experienced in their use; so fair, I have simply used Chromas Lite to quickly obtain a FASTA. I do not know nether how to use C, but I think this library somehow helps; just need to find a way to figure it out, thanks.

[Anima Mundi]

Thanks for the correction and sorry for the mistake, but I would have preferred to repair by my self the error, as you gave me this opportunity in the first 6 hours. Beside this, I appreciate the merging of wiee and Anima mundi but, again, I would have preferred to be consulted. I could appear demanding, I know, but if you want to be formal, in my opinion, you should offer first high standard. Sorry again for the frankness.