BLESS : ERROR: Irregular quality score range 35-75
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7.3 years ago
xzpgocxx ▴ 20

I get follows error output when used the bless to correct my Illumina paired-end reads, which I have trimmed used Trimmomatic software.

Checking input read files

ERROR: Irregular quality score range 35-75

--------------------------------------------------------------------------
MPI_ABORT was invoked on rank 0 in communicator MPI_COMM_WORLD 
with errorcode 103.

NOTE: invoking MPI_ABORT causes Open MPI to kill all MPI processes.
You may or may not see output from other processes, depending on
exactly when Open MPI kills them.
software error next-gen • 1.9k views
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Command-line used? BLESS version?

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Yes, command-line, VERSION: 1.02

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I meant the exact command-line you used.

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bless -read1 3k_R1.rd.clean.fastq -read2 3k_R2.rd.clean.fastq -prefix ../bless/3k_R12.rd.clean -kmerlength 31
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