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7.2 years ago
Pappu
★
2.1k
I am trying to convert transcript expression to gene expression. If I take mean, the results are skewed due to some low expressing transcripts.
Can you be a bit specific about it? If you are trying to summarize transcript to gene level for the expression from RNA-Seq then use tximport. Most of the pseudo aligners and quantification tools like Salmon/Kallisto have it employed as well. Taking mean is not the correct way.