Infer RNA-Seq library preparation from BAM
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7.2 years ago
ResearchR ▴ 120

Dear all,

Do you know a tool, which gives information, if a RNA-Seq library is poly-A or ribosomal depleted? I received a lot of RNA-Data, which has been sequenced in different labs with different libraries. I could check the RNA-SeQC output for the coverage at the 3' and 5' ends, but this will take forever. And yes, I already asked Google...

Thanks guys!

RNA-Seq NGS • 1.2k views
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I will assume your data is human RNAseq: find some non-poly-A lncRNAs with reasonable expression, them use those to infer library prep.

Or ask the person who organised all this data if he have this information as well.

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