Tools for Saturation analysis and gene body coverage analysis

Question

[Javad]

Hello,

I have a set of RNA-seq data and I want to perform saturation analysis and gene body coverage analysis on them. Which tools can I use for this type of analysis?

Thanks

Answer 1

[JY Nam]

For gene body coverage analysis, you can use RSeQC tool.

RSeQC

[Kristoffer Vitting-Seerup]

As for the saturation part of the analysis I think you have to do the subsampling for the bam file yourself.