Sorted scaffolds of a draft genome according to a reference genome
0
1
Entering edit mode
7.2 years ago
Mehmet ▴ 820

Dear all,

I would like to sorted scaffolds of a draft genome according to a reference genome. I used Mauve program. It reordered, but when I checked order of scaffolds of the draft genome, it seems that Mauve did not reorder scaffolds of the draft genome.

For instance, in first analysis (alignment of the genomes) scaffold 1 of the reference genome matched with scaffold 116 of the draft. When I reordered, the scaffold 116 did not replaced as the first scaffold of the reference genome.

I am using a worm species genomes for reordering.

Assembly alignment sequence genome • 1.8k views
ADD COMMENT
1
Entering edit mode

Have you tried MCM approach within Mauve? Sorting The Scaffolds After Assembling

ADD REPLY
0
Entering edit mode

Have you tried MCM approach within Mauve? Sorting The Scaffolds After Assembling

ADD REPLY
0
Entering edit mode

Yes, I did.

As I mentioned in my question;

"For instance, in first analysis (alignment of the genomes) scaffold 1 of the reference genome matched with scaffold 116 of the draft. When I reordered, the scaffold 116 did not replaced as the first scaffold of the reference genome".

What I want is to change position of scaffolds of the draft genome according to the alignment with the reference genome.

How can I do that?

ADD REPLY

Login before adding your answer.

Traffic: 1284 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6