Download Rrna Sequences
4
1
Entering edit mode
11.7 years ago

Hi,

Simple question : Where can I download all rRNA sequences (from human or other species). I found some of them using ensembl biomart but is there maybe other places to find them ?

Thanks

N.

rrna • 28k views
ADD COMMENT
4
Entering edit mode
11.7 years ago
vipin.ts ▴ 60

Go to http://central.biomart.org/ click on Ensembl, Restrict search Gene biotype as rRNA will give the organism specific list of rRNA regions in the genome.

ADD COMMENT
0
Entering edit mode

That's indeed the best solution. One thing to add: to download sequences you gotta click on the attribute and select sequence instead of regions.

ADD REPLY
3
Entering edit mode
11.7 years ago
User ▴ 70

The post was deleted.

ADD COMMENT
0
Entering edit mode

+1 on the link. Just a head's up on SILVA, it's a great resource (...and I've contributed to the database), but it's skewed toward microbial rRNA and not human. There are many Eukaryotes, but it's not "heavy" in the "human" sequence area.

ADD REPLY
1
Entering edit mode
ADD COMMENT
0
Entering edit mode

I am not sure this query will be specific enough to return only the rRNA sequenceS. I tried this : (rRNA[Feature key]) AND "Homo sapiens"[porgn:__txid9606] and see the first few results belong to whole chromosomes and not the rRNA sequences.

ADD REPLY
0
Entering edit mode

Are you looking at nuclear rRNA, mitochondrial rRNA, or both? Chromosome searching might be time consuming, but can you download all of the Human mitochondrial sequences you can find and parse out all the rRNA operons?

ADD REPLY
1
Entering edit mode
11.7 years ago
GAO Yang ▴ 250

Rfam database :a collection of RNA families http://rfam.sanger.ac.uk/

ADD COMMENT
0
Entering edit mode

Which database is better to work? SILVA or RFAM? For human, i found approx 200 sequences Large sub unit LSU+ sample sub unit SUU in SILVA database while in RFAM databases, sequences ranges from 2000-2100.

ADD REPLY

Login before adding your answer.

Traffic: 1571 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6