Entering edit mode
7.2 years ago
qudrat
▴
100
Hello folks! I have a transcript which is partial in length (obtained by TopHat/Cufflinks analysis) and I want to get its full length. Is there any way to get a full length transcript? Please suggest any Bioinformatics method.
Hi, could you please elaborate your issue, or maybe paste the partial sequences obtained?
blast it.
That could be done, but I was wondering if partial transcripts are generally obtained?
Actually Cufflinks assemble sequences parsimoniously so there is a chance to get partial sequence. The sequence I have got does not show any feature of 3'UTR. One more thing is that I have got a peptide sequence that map to chromosome 1 but my transcript is short of 100 bp. (peptide map 100 bp upstream of transcript first base). I have read many papers that have talked about partial assembly of transcript sequence.