Blast method to use for blasting genes against a transcriptome
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7.1 years ago

Hi,

I have a transcriptome assembly of a non-model organism for which I created a custom database using makeblastdb. I have the cDNA sequences of some of the genes I need to align with the transcriptome to find similar sequences. What blast strategy should I use for this? tblastn, blastx or simply blastn?

Thanks

RNA-Seq blast Assembly • 1.8k views
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7.1 years ago
Sej Modha 5.3k

I think blastn should do the job!

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Thanks! I will try blastn

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