can a barcode be present both in header and sequence
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7.1 years ago

Can a illumina sequence (HiSeq) has barcodes both in header and in sequence?

@J00113:305:HL5YGBBXX:8:1101:15483:1244 1:N:0::NAGGC
TATAGATGGAGGGATAGATGGAGGGCAGCCAGAATTTTTGAAGAAGTCATGTTTCACCAGCACAAAAATGAGTGAGTTACAGCCATGCAAAATGAGATGAAATCTTTGAATGAGAACCATAATTATGACTTAGTAAAGCTGCCTA
+
-A<FJFFJJFJJJA7FFFAJFJAFJ-7---777-<FJ-AFJFFFJJJ-<<-<<F-<--<--7-<77<7<FJJ7JJJFJJ-FF--7---<7A7F<FA7<7F-FA-777A-A-AFAJF--7-FAFF-F-7--7FFJJJ7<-77)))7
@J00113:305:HL5YGBBXX:8:1101:4137:1525 1:N:0::GAGGC
NAGGCATGATTTAATTTTTTATTTTTCATCATAACGCATAAAATGATGTTTTCTTTTACATTGAGAAAATATTTTTTCAATTACACTCTTTTAAATAGTAAAACTTATTTGTTTGACGGGTAATATTTTCAATCAATTCTCGCC
+
AA<AFJAFJFJJJJJAJJJAJJ<JJFJ-<F-<7JJ-<-<-<<FJ7FJ-J<FF--<<FJJ-<AFJJJJJJJJFAAJJFJ-<<7<--<---7<7FJJJJJ<A7FAJ-7A-7<<AJ<FAF77FAF7A7F<AFF-77F-7-77-7--))
@J00113:305:HL5YGBBXX:8:1101:4624:1525 1:N:0::GAGGC
TCATATTTTTGGCACACGGAGGGGCCTCGCAAAGTCTCACGCCATTCCGAGTCCGGCGGACGAACATCGCCAGCCCCCCGCCCCAAGGCTATAGCCCACCCGTTTTTTGGGCATTTTTCCGCTGGGGGGTTTTGGCGCCGCGACC
#AAA--A<FJFFJJFJJJA7FFFAJFJAFJ-7---777-<FJ-AFJFFFJJJ-<<-<<F-<--<--7-<77<7<FJJ7JJJFJJ-FF--7---<7A7F<FA7<7F-FA-777A-A-AFAJF--7-FAFF-F-7--7FFJJJ7<-77)))7
@J00113:305:HL5YGBBXX:8:1101:4137:1525 1:N:0:0
GAGGCTTAGGTATGATTTAATTTTTTATTTTTCATCATAACGCATAAAATGATGTTTTCTTTTACATTGAGAAAATATTTTTTCAATTACACTCTTTTAAATAGTAAAACTTATTTGTTTGACGGGTAATATTTTCAATCAATTCTCGCC
+
AAAF-AA<AFJAFJFJJJJJAJJJAJJ<JJFJ-<F-<7JJ-<-<-<<FJ7FJ-J<FF--<<FJJ-<AFJJJJJJJJFAAJJFJ-<<7<--<---7<7FJJJJJ<A7FAJ-7A-7<<AJ<FAF77FAF7A7F<AFF-77F-7-77-7--))
@J00113:305:HL5YGBBXX:8:1101:4624:1525 1:N:0:0
GAGGCTCATATTTTTGGCACACGGAGGGGCCTCGCAAAGTCTCACGCCATTCCGAGTCCGGCGGACGAACATCGCCAGCCCCCCGCCCCAAGGCTATAGCCCACCCGTTTTTTGGGCATTTTTCCGCTGGGGGGTTTTGGCGCCGCGACC

ls de-multiplexing with different ways and concatenating ok?

Thanks

next-gen • 1.2k views
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