Help with microarray annotation

Question

[fabbri.marco]

Dear all, I have received a tab separated file with probeset and thre column with gene expression. This matrix comes out from from old microarray study with SurePrint G3 Mouse Gene Expression v2 8x60K Microarray. I would like to add two extra column with gene annotation: GENE_ID and gene name. any quick suggestion? Thank you

Marco

the file is: pbobe sample1 smaple_2 lsampe_3 A_51_P414243 6.38237727640526 6.29565315710059 6.4258740045022 A_30_P01024440 6.80598849523631 6.83724951377773 7.32338042803567 A_30_P01025554 10.577830957849 10.1933730611416 10.4212913126594 A_51_P328014 8.40276381037392 8.07695764802267 8.30380783650057 A_30_P01019108 6.82976954176586 6.80979356921425 7.05726004244387 A_55_P2056220 8.71780606560799 8.86092849386466 8.8739000565048 A_55_P1985764 12.0240760134868 12.1324319131564 12.526274612236 A_52_P108321 6.74854175320155 6.69643630036016 6.82111120319708 A_52_P123354 8.56544085765343 8.66659180615821 8.73642604180768 A_55_P2061724 6.57505167765367 6.53739811458435 6.77440987839767 A_55_P2049122 8.04015846131788 7.34565904212156 8.37241743364832 A_55_P1988844 6.82691239881711 7.32088945656357 7.19212127016117 A_51_P385099 11.5598438645793 11.2884970242988 10.6401677172283 A_55_P2111005 9.09192187409387 8.70112454671877 9.21217765603996 A_55_P2041509 10.0284124247595 10.0390816566345 10.5839256764631 A_30_P01029765 6.89544008260402 6.90102263544552 6.94730016429129 A_51_P358243 7.00501292394617 7.2206341767886 6.8908009928329

Answer 1

[Shervin Alaei]

Hi, You should always look for the annotation file that usually comes with the utilized microarray platform.

In your case, a quick search for example in GEO, yields the following page with related files, which I think can solve your problem:

Platform GPL21163 A quick view of the annotation file with information such as 'probe_id', 'locuslink_id', 'gene_name', etc are displayed at the bottom of the page.