Non model organism(species) like planaria RNA SEQ analysis

Question

[hyojin0912]

Hi

I'm analyzing planaria RNA seq for regeneration study

But planaria genome is not well organized(Use SmedGD v3.1 fasta & GTF)

So I do blastx to get uniprotID but I'm having over 2,000 species' uniprot ID

Also, I have an assigned GO to each gene then I want to analyze at GO level

Is there any tool or paper which I can see...

Last time, I done GO enrichment test using EASE test (DAVID software) but I don't have enough number of GO to each gene.. this is also a problem of my analysis

Please help me anything

I'm looking forward to your reply

Thanks

[ashish]

easiest thing you can do is to upload your blast results into BLAST2GO. It has everything you are asking for.

[hyojin0912]

I already done B2G but blast ratio is too low..

[ashish]

you can try increasing the number of hits per query to consider but this would only work if you performed blastx using default parameters. If this doesn't help then then I think b2g cannot help you.