Using idba_un to read xxx.fa output into xxx_dir file, terminator showed invalid insert distance

Question

[wuziqi_94]

When I do the assembly, after merge xxx_1.fp and xxx_2.fp files into xxx_1.fa file, I used idba_un to read xxx_1.fa output into xxx_dir. One the screen, the terminator showed the invalid insert distance which was following the kmer command line. How to fix this problem? And what is the problems with invalid insert distance?

[Sej Modha]

Are you sure that the reads are properly paired and follows the same order in both fastq files prior to merging as that can cause such error in idba.

[wuziqi_94]

Thank you! I am sure that both two reads are properly paired and follow the same order in both fastq files. I am confused about how to fix the insert distance in IDBA to assembly the fastq files.